Cell division is essential for an organism to grow, but when a cell divides it must replicate the DNA in its genome so that the two daughter cells have the same genetic information as their parent. DNA provides a simple mechanism for replication. In transcription, or RNA synthesis, the codons of a gene are copied into messenger RNA by RNA polymerase.
As opposed to DNA replication, transcription results in an RNA complement that includes uracil (U) in all instances where thymine (T) would have occurred in a DNA complement.
|Purpose||The purpose of replication is to conserve the entire genome for next generation.||The purpose of transcription is to make RNA copies of individual genes that the cell can use in the biochemistry.|
|Definition||DNA replication is the replication of a strand of DNA into two daughter strands, each daughter strand contains half of the original DNA double helix.||Transcription is the synthesis of RNA from a DNA template.|
|Introduction (from Wikipedia)||Cell division is essential for an organism to grow, but when a cell divides it must replicate the DNA in its genome so that the two daughter cells have the same genetic information as their parent.DNA provides a simple mechanism for replication.||In transcription, the codons of a gene are copied into messenger RNA by RNA polymerase|
|Products||One strand of DNA becomes 2 daughter strands.||mRNA, tRNA, rRNA and non-coding RNA( like microRNA)|
|Base Pairing||Since there are 4 bases in 3-letter combinations, there are 64 possible codons (43 combinations).||RNA transcription follows base pairing rules. The enzyme makes the complementary strand by finding the correct base through complementary base pairing, and bonding it onto the original strand.|
|Product processing||In eukaryotes complementary base pair nucleotides bond with the sense or antisense strand. Thesre are then connected with phosphoester bonds by DNA helix to create a complete strand.||A 5’ cap is added, a 3’ poly A tail is added and introns are spliced out.|
|Codons||These encode the twenty standard amino acids, giving most amino acids more than one possible codon. There are also three 'stop' or 'nonsense' codons signifying the end of the coding region; these are the UAA, UAG and UGA codons.||DNA polymerases can only extend a DNA strand in a 5′ to 3′ direction, different mechanisms are used to copy the antiparallel strands of the double helix. In this way, the base on the old strand dictates which base appears on the new strand.|
|Result||In replication, the end result is two daughter cells.||while in transcription, the end result is a RNA molecule.|
|Methods to measure and detect||Meselson and Stahl Experiment||RT-PCR, DNA microarray, In-situ hybridization, Northern blot, RNA-Seq.|
|Product||Replication is the duplication of two-strands of DNA.||Transcription is the formation of single, identical RNA from the two-stranded DNA.|
|Enzymes||The two strands are separated and then each strand's complementary DNA sequence is recreated by an enzyme called DNA polymerase.||In transcription, the codons of a gene are copied into messenger RNA by RNA polymerase.This RNA copy is then decoded by a ribosome that reads the RNA sequence by base-pairing the messenger RNA to transfer RNA, which carries amino acids.|
|Enzymes Required||DNA Helicase, DNA Polymerase.||Transcriptase (type of DNA Helicase), RNA polymerase.|
edit Video Explaining the Differences
The DNA replication and mRNA transcription process are explained in the following video. Notice that while explaining about DNA replication, it also touches on the process of mutation.